Validation Data Gallery
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Tested Applications
| Positive WB detected in | HUVEC cells, K-562 cells, RT-4 cells, SiHa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31297-1-AP targets CDC42BPA in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDC42BPA fusion protein Ag34955 相同性解析による交差性が予測される生物種 |
| Full Name | CDC42 binding protein kinase alpha (DMPK-like) |
| Calculated molecular weight | 197 kDa |
| Observed molecular weight | 200 kDa |
| GenBank accession number | NM_001366019 |
| Gene Symbol | CDC42BPA |
| Gene ID (NCBI) | 8476 |
| RRID | AB_3669934 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q5VT25 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cdc42 binding protein kinase alpha (Cdc42BPA/MRCKα), is a member of the serine/threonine protein kinase family. Previous studies suggested that Cdc42BPA may mediates the Cdc42induced peripheral actin formation to promote cytoskeletal reorganization 5. However, the biological function and clinical significance of Cdc42BPA in colon cancer are unclear. (PMID: 29072916)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC42BPA antibody 31297-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |