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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDC42 Monoclonal antibody (67212-1-Ig)

    WB analysis using 67212-1-Ig (same clone as 67212-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CDC42 Monoclonal antibody (67212-1-Ig)

    WB analysis using 67212-1-Ig (same clone as 67212-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using CDC42 Monoclonal antibody (67212-1-Ig)

    WB analysis of mouse cerebellum using 67212-1-Ig (same clone as 67212-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67212-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CDC42 Monoclonal antibody (67212-1-Ig)

    WB analysis using 67212-1-Ig (same clone as 67212-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67212-1-Ig (CDC42 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67212-1-PBS in a different storage buffer formulation.

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Tested Applications

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67212-1-PBS targets CDC42 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen CDC42 fusion protein Ag28550 相同性解析による交差性が予測される生物種
Full Name cell division cycle 42 (GTP binding protein, 25kDa)
Calculated molecular weight 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC002711
Gene Symbol CDC42
Gene ID (NCBI) 998
RRIDAB_2918484
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP60953
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking