Validation Data Gallery
Tested Applications
| Positive WB detected in | Y79 cells, mouse brain tissue |
| Positive IHC detected in | mouse testis tissue, rat stomach tissue, rat testis tissue, mouse liver tissue, human stomach cancer tissue, mouse stomach tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
13761-1-AP targets CDK8/19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4731 Product name: Recombinant human CDC2L6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-335 aa of BC037289 Sequence: MDYDFKAKLAAERERVEDLFEYEGCKVGRGTYGHVYKARRKDGKDEKEYALKQIEGTGISMSACREIALLRELKHPNVIALQKVFLSHSDRKVWLLFDYAEHDLWHIIKFHRASKANKKPMQLPRSMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPFHHDQLDRIFSVMGFPADKDWEDIRKMPEYPTLQKDFRRTTYANSSLIKYMEKHKVKPDSKVFLLLQKLLTMDPTKRITSEQALQDPYF 相同性解析による交差性が予測される生物種 |
| Full Name | cell division cycle 2-like 6 (CDK8-like) |
| Calculated molecular weight | 502 aa, 57 kDa |
| Observed molecular weight | 50-57 kDa |
| GenBank accession number | BC037289 |
| Gene Symbol | CDK19 |
| Gene ID (NCBI) | 23097 |
| RRID | AB_2276048 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BWU1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The CDK8 homolog, CDK19 (Cdk11, CDC2L6) was discovered by Hidden Markov model (HMM) profiling of a eukaryotic protein kinase domain, and found to have approximately 91% sequence homology to Cdk8. The two proteins share a particularly high degree of sequence conservation in their kinase and cyclin binding domain (PMID: 30693281, 26985305). CDK8 and CDK19, collectively termed 'Mediator Kinase,' are incorporated, in a mutually exclusive manner, as part of a 4-protein complex called the Mediator kinase module. CDK8/19 inhibition suppressed the induction of NFκB-driven transcription by p21 (PMID: 28855340).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CDK8/19 antibody 13761-1-AP | Download protocol |
| WB protocol for CDK8/19 antibody 13761-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Characterization of gRNA-dependent and gRNA-independent off-target binding sites of PspCas13b and RfxCas13d in mammalian cells. | ||
J Med Chem Optimized CDK19 PET Tracers: Spirocyclic-Driven Design and Prostate Cancer Imaging. | ||
Cell Mol Life Sci Hotair promotes the migration and proliferation in ovarian cancer by miR-222-3p/CDK19 axis. | ||
Fitoterapia Phytochemistry analysis of the above-ground parts of Sanguisorba officinalis L. and its effects on myocardial cell metabolism |