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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    WB analysis of Jurkat using 67239-1-Ig (same clone as 67239-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    WB analysis of HSC-T6 using 67239-1-Ig (same clone as 67239-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    WB analysis of 4T1 using 67239-1-Ig (same clone as 67239-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    WB analysis of HEK-293 using 67239-1-Ig (same clone as 67239-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IHC staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IHC staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IF Staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IF Staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IF Staining of HEK-293 using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    FC experiment of K-562 using 67239-1-Ig (same clone as 67239-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CDC27; APC3 (67239-1-Ig, Clone:2F10G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

CDC27; APC3 Monoclonal antibody, PBS Only

CDC27; APC3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 67239-1-PBS
Clone No.2F10G2

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

CDC27, CDC27 homolog, APC3, APC 3, Anaphase-promoting complex subunit 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67239-1-PBS targets CDC27; APC3 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25588

Product name: Recombinant human CDC27; APC3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 481-830 aa of BC011656

Sequence: ALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

相同性解析による交差性が予測される生物種
Full Name cell division cycle 27 homolog (S. cerevisiae)
Calculated molecular weight 92 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC011656
Gene Symbol CDC27
Gene ID (NCBI) 996
RRIDAB_2882518
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP30260
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.

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