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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDC25C Monoclonal antibody, PBS Only

CDC25C Monoclonal Antibody for WB, FC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B6B12

Cat no : 66912-1-PBS

Synonyms

CDC25, CDC25C, M phase inducer phosphatase 3

Validation Data Gallery

Applications:
  • WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

    WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

  • WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

    WB analysis using 66912-1-Ig (same clone as 66912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 66912-1-Ig (same clone as 66912-1-PBS)

    FC experiment of K-562 using 66912-1-Ig (same clone as 66912-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human CDC25C (66912-1-Ig, Clone:2B6B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH . This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66912-1-PBS targets CDC25C in WB, FC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDC25C fusion protein Ag10240 相同性解析による交差性が予測される生物種
Full Name cell division cycle 25 homolog C (S. pombe)
Calculated molecular weight 473 aa, 53 kDa
Observed molecular weight 46-53 kDa
GenBank accession numberBC019089
Gene symbol CDC25C
Gene ID (NCBI) 995
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.