Validation Data Gallery
Tested Applications
| Positive WB detected in | SKOV-3 cells, HaCaT cells, A-204 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88426-1-RR targets CDC25B in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0994 Product name: Recombinant human CDC25B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC006395 Sequence: MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLGSHGLLGSPVRAAASSPVTTLTQTMHDLAGLGSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKT 相同性解析による交差性が予測される生物種 |
| Full Name | cell division cycle 25 homolog B (S. pombe) |
| Calculated molecular weight | 65 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC006395 |
| Gene Symbol | CDC25B |
| Gene ID (NCBI) | 994 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P30305 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDC25B, also named CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase that functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC25B antibody 88426-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |