Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue, Jurkat cells, mouse brain tissue, Raji cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 2 publications below |
Product Information
10644-1-AP targets CDC25B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDC25B fusion protein Ag0994 相同性解析による交差性が予測される生物種 |
| Full Name | cell division cycle 25 homolog B (S. pombe) |
| Calculated molecular weight | 65 kDa |
| Observed molecular weight | 60-70 kDa |
| GenBank accession number | BC006395 |
| Gene Symbol | CDC25B |
| Gene ID (NCBI) | 994 |
| RRID | AB_2075439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30305 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC25B antibody 10644-1-AP | Download protocol |
| IHC protocol for CDC25B antibody 10644-1-AP | Download protocol |
| IP protocol for CDC25B antibody 10644-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Dev Biol Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation. | ||
BMC Dev Biol YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation. |