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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDC25A Monoclonal antibody (68457-1-Ig)

    WB analysis using 68457-1-Ig (same clone as 68457-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68457-1-Ig (CDC25A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68457-1-PBS in a different storage buffer formulation.

CDC25A Monoclonal antibody, PBS Only

CDC25A Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68457-1-PBS
Clone No.3C3A8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

3C3A8, CDC25, CDC25A2, EC:3.1.3.48, M-phase inducer phosphatase 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68457-1-PBS targets CDC25A in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cell division cycle 25 homolog A (S. pombe)
Calculated molecular weight 59 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberNM_001789
Gene Symbol CDC25A
Gene ID (NCBI) 993
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP30304
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.