Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
13376-1-AP targets CDC20B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4198 Product name: Recombinant human CDC20B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-192 aa of BC037547 Sequence: MEWKLERTAPRRVRTEEEMLWVLDSVNATYSDFKSNFAKRLSAEVPVASSPITTRWQQSQTRALSSDSFGEEQSTTYLPEASGSVLKTPPEKETLTLGSCKEQLKTPSKGISETSNSALHFCKAPHAMDRDWKESVASKGQKCLKQLFVTQNVVQQANGKMQLCEQSECVWKDHFSGSMKKRFEQEDVGGKD 相同性解析による交差性が予測される生物種 |
| Full Name | cell division cycle 20 homolog B (S. cerevisiae) |
| Calculated molecular weight | 192 aa, 22 kDa |
| GenBank accession number | BC037547 |
| Gene Symbol | CDC20B |
| Gene ID (NCBI) | 166979 |
| RRID | AB_2877945 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86Y33 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CDC20B antibody 13376-1-AP | Download protocol |
| IF protocol for CDC20B antibody 13376-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun CDC20B is required for deuterosome-mediated centriole production in multiciliated cells. | ||
Front Oncol Identification of Hub Genes Associated With Melanoma Development by Comprehensive Bioinformatics Analysis. | ||
Bioengineered Circular RNA hsa_circ_0008896 accelerates atherosclerosis by promoting the proliferation, migration and invasion of vascular smooth muscle cells via hsa-miR-633/CDC20B (cell division cycle 20B) axis. |