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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    WB analysis using 14307-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    WB analysis of NIH/3T3 using 14307-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    WB analysis of HeLa using 14307-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    WB analysis of K-562 using 14307-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    IHC staining of human cervical cancer using 14307-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    IHC staining of human cervical cancer using 14307-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    IF Staining of HeLa using 14307-1-AP

    Immunofluorescent analysis of Hela cells, using CDC16 antibody 14307-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

CDC16/APC6 Polyclonal antibody

CDC16/APC6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14307-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CDC16, APC6, CDC16Hs, CDC16 homolog, Anaphase-promoting complex subunit 6

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, NIH/3T3 cells, Jurakt cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14307-1-AP targets CDC16/APC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5427

Product name: Recombinant human CDC16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 178-527 aa of BC010875

Sequence: IGTLVELNKANELFYLSHKLVDLYPSNPVSWFAVGCYYLMVGHKNEHARRYLSKATTLEKTYGPAWIAYGHSFAVESEHDQAMAAYFTAAQLMKGCHLPMLYIGLEYGLTNNSKLAERFFSQALSIAPEDPFVMHEVGVVAFQNGEWKTAEKWFLDALEKIKAIGNEVTVDKWEPLLNNLGHVCRKLKKYAEALDYHRQALVLIPQNASTYSAIGYIHSLMGNFENAVDYFHTALGLRRDDTFSVTMLGHCIEMYIGDSEAYIGADIKDKLKCYDFDVHTMKTLKNIISPPWDFREFEVEKQTAEETGLTPLETSRKTPDSRPSLEETFEIEMNESDMMLETSMSDHST

相同性解析による交差性が予測される生物種
Full Name cell division cycle 16 homolog (S. cerevisiae)
Calculated molecular weight 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC010875
Gene Symbol CDC16/APC6
Gene ID (NCBI) 8881
RRIDAB_2074638
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13042
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CDC16/APC6 antibody 14307-1-APDownload protocol
IHC protocol for CDC16/APC6 antibody 14307-1-APDownload protocol
WB protocol for CDC16/APC6 antibody 14307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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