Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
17597-1-AP targets CDAN1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDAN1 fusion protein Ag11589 相同性解析による交差性が予測される生物種 |
| Full Name | congenital dyserythropoietic anemia, type I |
| Calculated molecular weight | 1227 aa, 134 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC066640 |
| Gene Symbol | CDAN1 |
| Gene ID (NCBI) | 146059 |
| RRID | AB_10694142 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IWY9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDAN1 acts as a negative regulator of ASF1 in chromatin assembly. It appears to play a role in nuclear envelope integrity, possibly related to microtubule attachments. Mutations of CDAN1 cause congenital dyserythropoietic anemia type I, a disease resulting in morphological and functional abnormalities of erythropoiesis. There're some isoforms this gene.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDAN1 antibody 17597-1-AP | Download protocol |
| IF protocol for CDAN1 antibody 17597-1-AP | Download protocol |
| IP protocol for CDAN1 antibody 17597-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem J A complex comprising C15ORF41 and Codanin-1: the products of two genes mutated in congenital dyserythropoietic anaemia type I (CDA-I). |