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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human testis tissue using CDADC1 Polyclonal antibody (28169-1-AP)

    IHC staining of human testis using 28169-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 28169-1-AP (CDADC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CDADC1 Polyclonal antibody (28169-1-AP)

    IHC staining of mouse testis using 28169-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28169-1-AP (CDADC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using CDADC1 Polyclonal antibody (28169-1-AP)

    IF Staining of Saos-2 using 28169-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using CDADC1 antibody (28169-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using CDADC1 Polyclonal antibody (28169-1-AP)

    IF Staining of Saos-2 using 28169-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using CDADC1 antibody (28169-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using CDADC1 Polyclonal antibody (28169-1-AP)

    IF Staining of Saos-2 using 28169-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using CDADC1 antibody (28169-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

CDADC1 Polyclonal antibody

CDADC1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 28169-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

bA103J18.1, CDADC1, NYD SP15

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSaos-2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28169-1-AP targets CDADC1 in IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27374

Product name: Recombinant human CDADC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-188 aa of BC125202

Sequence: PAEAQRQKSQKNEEGKHGPLGDNEERTRVSTDKRQVKRTGLVVVKNMKIVGLHCSSEDLHAGQIALIKHGSRLKNCDLYFSRKPCSACLKMIVNAGVNRISYWPADPEISLLTEASSSEDAKLDAKAVERLKSNSRAHV

相同性解析による交差性が予測される生物種
Full Name cytidine and dCMP deaminase domain containing 1
Calculated molecular weight 514 aa, 58 kDa
GenBank accession numberBC125202
Gene Symbol CDADC1
Gene ID (NCBI) 81602
RRIDAB_3086034
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BWV3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDADC1 (cytidine and dCMP deaminase domain containing 1) catalyzes the deamination of cytidine and deoxycytidine into uridine and deoxyuridine, respectively, and may play an important role in testicular development and spermatogenesis (PMID: 26945630, 16955368).

Protocols

Product Specific Protocols
IF protocol for CDADC1 antibody 28169-1-APDownload protocol
IHC protocol for CDADC1 antibody 28169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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