Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 66883-1-Ig (same clone as 66883-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66883-1-Ig (same clone as 66883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 66883-1-Ig (same clone as 66883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66883-1-Ig (same clone as 66883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66883-1-Ig (same clone as 66883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66883-1-Ig (same clone as 66883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 66883-1-Ig (same clone as 66883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 66883-1-Ig (same clone as 66883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66883-1-Ig (same clone as 66883-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66883-1-Ig (same clone as 66883-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CD98/SLC3A2 antibody (66883-1-Ig, Clone: 2B10F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

CD98/SLC3A2 Monoclonal antibody, PBS Only

CD98/SLC3A2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66883-1-PBS

Clone No.2B10F5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD98, SLC3A2, 2B10F5, 4F2 cell-surface antigen heavy chain, Amino acid transporter heavy chain SLC3A2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66883-1-PBS targets CD98/SLC3A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag7301 Product name: Recombinant human SLC3A2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 269-630 aa of BC001061 Sequence: KDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Calculated molecular weight	68 kDa
Observed molecular weight	85-94 kDa, 120-130 kDa
GenBank accession number	BC001061
Gene Symbol	CD98
Gene ID (NCBI)	6520
RRID	AB_2882215
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P08195
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

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Species Reactivity

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Conjugate

Validation Data Gallery

WB analysis using 66883-1-Ig (same clone as 66883-1-PBS)

IHC staining of human renal cell carcinoma using 66883-1-Ig (same clone as 66883-1-PBS)