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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD98/SLC3A2 Recombinant antibody (84387-6-RR)

    WB analysis using 84387-6-RR (same clone as 84387-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84387-6-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84387-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CD98/SLC3A2 Recombinant antibody (84387-6-RR)

    IF Staining of HepG2 using 84387-6-RR (same clone as 84387-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD98 antibody (84387-6-RR, Clone: 241755A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84387-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CD98/SLC3A2 Recombinant antibody (84387-6-RR)

    IF Staining of A431 using 84387-6-RR (same clone as 84387-6-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD98/SLC3A2 antibody (84387-6-RR, Clone: 241755A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84387-6-PBS in a different storage buffer formulation.

CD98/SLC3A2 Recombinant monoclonal antibody, PBS Only

CD98/SLC3A2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84387-6-PBS
Clone No.241755A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CD98, SLC3A2, 241755A11, 4F2 cell-surface antigen heavy chain, Amino acid transporter heavy chain SLC3A2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84387-6-PBS targets CD98/SLC3A2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1948

Product name: Recombinant Human CD98/SLC3A2 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 206-630 aa of NM_002394.5

Sequence: RAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA

相同性解析による交差性が予測される生物種
Full Name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Calculated molecular weight68 kDa
Observed molecular weight68-125 kDa
GenBank accession numberNM_002394.5
Gene Symbol CD98
Gene ID (NCBI) 6520
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP08195-1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD98 is a 125-kDa type II membrane glycoprotein heterodimer composed of a 40-kDa non-glycosylated light chain (amino-acid transporter) and an 85-kDa glycosylated heavy chain (CD98hc) (PMID: 11507094). CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). Molecular orientation studies have demonstrated that the C-terminal tail of human CD98 (hCD98), which contains a PDZ-binding domain, is extracellular (PMID: 18625289).