Validation Data Gallery
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Tested Applications
| Positive WB detected in | A375 cells, MOLT-4 cells, HuT 78 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85439-7-RR targets CD96 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CD96 fusion protein Eg3520 相同性解析による交差性が予測される生物種 |
| Full Name | CD96 molecule |
| Calculated molecular weight | 402 aa, 46 kDa |
| Observed molecular weight | 160 |
| GenBank accession number | NM_198196 |
| Gene Symbol | CD96 |
| Gene ID (NCBI) | 10225 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P40200 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD96 is a member of the poliovirus receptor (PVR, CD155)-nectin family that includes T cell Ig and ITIM domain (TIGIT) and CD226.. CD96 expression is broadly similar between mice and humans, and is present on a proportion of hematopoietic stem cells, αβ and γδ T-cells, NK cells and a sub-population of B-cells in humans and present on αβ and γδ T-cells, NK cells and NKT cells in mice (PMID: 32043568, 29531070)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD96 antibody 85439-7-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |