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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD94 Recombinant antibody (84466-4-RR)

    WB analysis using 84466-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84466-4-RR (CD94 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84466-4-RR

    Affinity and Kinetic Characterization of 84466-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84466-4-RR against Human CD94 were performed. The affinity constant is 3.62 nM.

CD94 Recombinant monoclonal antibody

CD94 Recombinant Antibody for WB, ELISA
Cat No. 84466-4-RR
Clone No.241704B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

NK cell receptor, KP43, KLRD1, Killer cell lectin-like receptor subfamily D member 1, 241704B5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inNK-92 cells, K-562 cells, SH-SY5Y cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84466-4-RR targets CD94 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Recombinant Protein

相同性解析による交差性が予測される生物種
Full Name killer cell lectin-like receptor subfamily D, member 1
Calculated molecular weight 179 aa, 20 kDa
Observed molecular weight43 kDa
GenBank accession numberBC028009
Gene Symbol CD94
Gene ID (NCBI) 3824
RRIDAB_3671989
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ13241
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD94, also known as KLRD1, is a membrane protein that is preferentially expressed on NK cells. CD94 can form disulfide-bonded heterodimers with NKG2 family members. The CD94/NKG2A heterodimer constitutes an inhibitory receptor that recognizes HLA-E.

Protocols

Product Specific Protocols
WB protocol for CD94 antibody 84466-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols