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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD90.1 Recombinant antibody (84832-1-RR)

    WB analysis using 84832-1-RR (same clone as 84832-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84832-1-RR (CD90.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD90.1 Recombinant antibody (84832-1-RR)

    IHC staining of mouse spleen using 84832-1-RR (same clone as 84832-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84832-1-RR (CD90.1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD90.1 Recombinant antibody (84832-1-RR)

    IHC staining of mouse spleen using 84832-1-RR (same clone as 84832-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84832-1-RR (CD90.1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD90.1 Recombinant antibody (84832-1-RR)

    IF Staining of mouse spleen using 84832-1-RR (same clone as 84832-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD90.1 antibody (84832-1-RR, Clone: 241542D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84832-1-RR

    Affinity and Kinetic Characterization of 84832-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84832-1-RR against Mouse CD90.1 were performed. The affinity constant is 17.0 nM.

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Mouse CD90.1 Recombinant antibody, PBS Only

CD90.1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84832-1-PBS
Clone No.241542D7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CD90, Thy1, Thy 1, 241542D7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84832-1-PBS targets CD90.1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name thymus cell antigen 1, theta
Observed molecular weight25 kDa
Gene Symbol CD90
Gene ID (NCBI) 21838
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR and FVB (PMID: 5919593).