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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Multi-rAb™ CD9 Multi-Recombinant antibody (RMX00018)

    WB analysis using RMX00018

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00018 (CD9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Multi-rAb™ CD9 Multi-Recombinant antibody (RMX00018)

    IHC staining of human ovary cancer using RMX00018

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using RMX00018 (CD9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Multi-rAb™ CD9 Multi-Recombinant antibody (RMX00018)

    IF Staining of MCF-7 using RMX00018

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (RMX00018) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Multi-rAb™ CD9 Multi-Recombinant antibody

CD9 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00018

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD9 molecule, CD9 antigen, BTCC 1, BA2, 5H9 antigen

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, L02 cells, human placenta tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00018 targets CD9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD9 molecule
Calculated molecular weight25 kDa
Observed molecular weight 23-30 kDa
GenBank accession numberNM_001769.4
Gene Symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form Liquid
Purification MethodN/A
UNIPROT IDP21926
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).

Protocols

Product Specific Protocols
WB protocol for CD9 antibody RMX00018Download protocol
IHC protocol for CD9 antibody RMX00018Download protocol
IF protocol for CD9 antibody RMX00018Download protocol
Standard Protocols
Click here to view our Standard Protocols