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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood platelets using PE Anti-Human CD9 (MM2/57) (PE-65070)

    FC experiment of human peripheral blood platelets using PE-65070

    1X10^6 human peripheral blood platelets were surface stained with 10 ul PE Anti-Human CD9 (PE-65070, Clone:MM2/57) (red) or PE-mouse IgG2b isotype control (black). Cells were not fixed.

PE Anti-Human CD9 (MM2/57)

CD9 Monoclonal Antibody for FC
Cat No. PE-65070
Clone No.MM2/57

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9 antigen

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size/Concentration: 

-/ -

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Tested Applications

Positive FC detected inhuman peripheral blood platelets

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 10 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65070 targets CD9 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

N/A

相同性解析による交差性が予測される生物種
Full Name CD9 molecule
Calculated molecular weight 228 aa, 25 kDa
GenBank accession numberBC011988
Gene Symbol CD9
Gene ID (NCBI) 928
RRIDAB_2883867
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form
FormLiquid
Purification MethodAffinity purification
UNIPROT IDP21926
Storage Buffer PBS with 0.1% sodium azide and a stabilizer{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).

Protocols

Product Specific Protocols
FC protocol for PE CD9 antibody PE-65070Download protocol
Standard Protocols
Click here to view our Standard Protocols