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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD9 Recombinant antibody (82105-1-RR)

    WB analysis using 82105-1-RR (same clone as 82105-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:24370 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human urine exosomes tissue using CD9 Recombinant antibody (82105-1-RR)

    WB analysis of human urine exosomes using 82105-1-RR (same clone as 82105-1-PBS)

    human urine and human urine-derived exosomes were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD9 Recombinant antibody (82105-1-RR)

    IHC staining of human lung cancer using 82105-1-RR (same clone as 82105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD9 Recombinant antibody (82105-1-RR)

    IHC staining of human lung cancer using 82105-1-RR (same clone as 82105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using CD9 Recombinant antibody (82105-1-RR)

    IHC staining of human endometrial cancer using 82105-1-RR (same clone as 82105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using CD9 Recombinant antibody (82105-1-RR)

    IHC staining of human endometrial cancer using 82105-1-RR (same clone as 82105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 82105-1-RR (CD9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CD9 Recombinant antibody (82105-1-RR)

    IF Staining of MCF-7 using 82105-1-RR (same clone as 82105-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (82105-1-RR, Clone: 5K14 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 82105-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82105-1-RR

    Affinity and Kinetic Characterization of 82105-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82105-1-RR against Human CD9 were performed. The affinity constant is 36.3 pM.

CD9 Recombinant monoclonal antibody, PBS Only

CD9 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82105-1-PBS
Clone No.5K14

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CD9 antigen, BTCC 1, BA2, 5K14, 5H9 antigen

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82105-1-PBS targets CD9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag14546

Product name: Recombinant human CD9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-208 aa of BC011988

Sequence: FAIEIAAAIWGYSHKDEVIKEVQEFYKDTYNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHIIGAVGIGIAVVMI

相同性解析による交差性が予測される生物種
Full Name CD9 molecule
Calculated molecular weight 228 aa, 25 kDa
Observed molecular weight23-27 kDa
GenBank accession numberBC011988
Gene Symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP21926
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and support myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in fertilization of mammals (PMID: 25536312).

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