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  • Featured Product
  • KD/KO Validated

CD9 Monoclonal antibody, PBS Only

CD9 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H7B9

Cat no : 60232-1-PBS

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, Cell and organelle markers, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 60232-1-Ig (same clone as 60232-1-PBS)

    WB analysis of HeLa using 60232-1-Ig (same clone as 60232-1-PBS)

    WB result of CD9 antibody (60232-1-Ig, 1:2000) with si-Control and si-CD9 transfected HeLa cells. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • WB analysis using 60232-1-Ig (same clone as 60232-1-PBS)

    WB analysis using 60232-1-Ig (same clone as 60232-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60232-1-Ig (CD9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human colon cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    IHC staining of human colon cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human colon cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    IHC staining of human colon cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    IHC staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    IHC staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human tonsillitis using 60232-1-Ig (same clone as 60232-1-PBS)

    IHC staining of human tonsillitis using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IHC staining of human tonsillitis using 60232-1-Ig (same clone as 60232-1-PBS)

    IHC staining of human tonsillitis using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD9 antibody included
  • IF Staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    IF Staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • IF Staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    IF Staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    IF Staining of human lung cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    IF Staining of human lung cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 60232-1-Ig (same clone as 60232-1-PBS)

    FC experiment of HeLa using 60232-1-Ig (same clone as 60232-1-PBS)

    1X10^6 HeLa cells were stained with 0.2 ug Anti-Human CD9 (60232-1-Ig, Clone:4H7B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or 0.2 ug isotype control (black). Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy CD9 Ready-To-Use IHC Kit. CD9 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

60232-1-PBS targets CD9 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD9 fusion protein Ag14529 相同性解析による交差性が予測される生物種
Full Name CD9 molecule
Calculated molecular weight 228 aa, 25 kDa
Observed molecular weight 23-27 kDa
GenBank accession numberBC011988
Gene symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.