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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD8a Polyclonal antibody (33186-1-AP)

    WB analysis using 33186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33186-1-AP (CD8a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD8a Polyclonal antibody (33186-1-AP)

    IHC staining of rat spleen using 33186-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 33186-1-AP (CD8a antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD8a Polyclonal antibody (33186-1-AP)

    IHC staining of rat spleen using 33186-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 33186-1-AP (CD8a antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat thymus tissue using CD8a Polyclonal antibody (33186-1-AP)

    IHC staining of rat thymus using 33186-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 33186-1-AP (CD8a antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat thymus tissue using CD8a Polyclonal antibody (33186-1-AP)

    IHC staining of rat thymus using 33186-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 33186-1-AP (CD8a antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CD8a Polyclonal antibody

CD8a Polyclonal Antibody for WB, IHC, ELISA
Cat No. 33186-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CD8, CD8 antigen 32 kDa chain, CD8a molecule, OX-8 membrane antigen, T-cell surface glycoprotein CD8 alpha chain

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse thymus tissue, rat spleen tissue
Positive IHC detected inrat spleen tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

33186-1-AP targets CD8a in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD8a fusion protein Eg3839 相同性解析による交差性が予測される生物種
Full Name CD8a molecule
Calculated molecular weight26 kDa
Observed molecular weight30-34 kDa
GenBank accession numberNM_031538.2
Gene Symbol Cd8a
Gene ID (NCBI) 24930
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP07725
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
WB protocol for CD8a antibody 33186-1-APDownload protocol
IHC protocol for CD8a antibody 33186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols