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Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD86 (GL1) (CL647-65068)

    FC experiment of mouse splenocytes using CL647-65068

    1x10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD86 (GL1) (CL647-65068, Clone: GL1). Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using CoraLite® Plus 647 Anti-Mouse CD86 (GL1) (CL647-65068)

    FC experiment of mouse splenocytes using CL647-65068

    1x10^6 LPS-treated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD86 (GL1) (CL647-65068, Clone: GL1)(red) or CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD86 (GL1) (CL647-65068, Clone: GL1) (black, dashed). Cells were not fixed.

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CoraLite® Plus 647 Anti-Mouse CD86 (GL1)

CD86 Monoclonal Antibody for FC
Cat No. CL647-65068
Clone No.GL1

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Synonyms

B7, B7 2, B7.2, B70, Cd28l2, Cd86, CD86 antigen, CLS1, ETC 1, Ly 58, Ly58, MB7, MB7 2, TS/A 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inLPS treated mouse splenocytes

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65068 targets CD86 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen LPS-activated CBA/Ca mouse spleen B cells 相同性解析による交差性が予測される生物種
Full Name CD86 antigen
GenBank accession numberBC013807
Gene Symbol Cd86
Gene ID (NCBI) 12524
RRIDAB_2883663
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP42082
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD86 antibody CL647-65068Download protocol
Standard Protocols
Click here to view our Standard Protocols