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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CD84 Polyclonal antibody (12566-1-AP)

    WB analysis of Raji using 12566-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 12566-1-AP (CD84 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CD84 Polyclonal antibody (12566-1-AP)

    WB analysis of mouse thymus using 12566-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12566-1-AP (CD84 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using CD84 Polyclonal antibody (12566-1-AP)

    WB analysis of mouse liver using 12566-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12566-1-AP (CD84 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CD84 Polyclonal antibody (12566-1-AP)

    WB analysis of U-937 using 12566-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 12566-1-AP (CD84 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using CD84 Polyclonal antibody (12566-1-AP)

    WB analysis of mouse spleen using 12566-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12566-1-AP (CD84 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

CD84 Polyclonal antibody

CD84 Polyclonal Antibody for WB, ELISA
Cat No. 12566-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CD84 molecule, Cell surface antigen MAX.3, hCD84, Hly9 beta, Hly9-beta

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, mouse liver tissue, mouse spleen tissue, mouse thymus tissue, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12566-1-AP targets CD84 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3192

Product name: Recombinant human CD84 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-328 aa of BC020063

Sequence: DSEIFTVNGILGESVTFPVNIQEPRQVKIIAWTSKTSVAYVTPGDSETAPVVTVTHRNYYERIHALGPNYNLVISDLRMEDAGDYKADINTQADPYTTTKRYNLQIYRRLGKPKITQSLMASVNSTCNVTLTCSVEKEEKNVTYNWSPLGEEGNVLQIFQTPEDQELTYTCTAQNPVSNNSDSISARQLCADIAMGFRTHHTGLLSVLAMFFLLVLILSSVFLFRLFKRRQDAASKKTIYTYIMASRNTQPAESRIYDEILQSKVLPSKEEPVNTVYSEVQFADKMGKASTQDSKPPGTSSYEIVI

相同性解析による交差性が予測される生物種
Full Name CD84 molecule
Calculated molecular weight 345 aa, 39 kDa
Observed molecular weight 45 kDa, 66-70 kDa
GenBank accession numberBC020063
Gene Symbol CD84
Gene ID (NCBI) 8832
RRIDAB_10638482
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CD84 antibody 12566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Arthritis Rheum

Decreased microRNA-142-3p/5p expression causes CD4+ T cell activation and B cell hyperstimulation in systemic lupus erythematosus.

Authors - Ding Shu S
View Article
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