Validation Data Gallery
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Tested Applications
| Positive FC detected in | Human monocytes-derived dendritic cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 15 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65171 targets CD83 in FC applications and shows reactivity with human, non-human primates samples.
| Tested Reactivity | human, non-human primates |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Human CD83-transfected Cos cells 相同性解析による交差性が予測される生物種 |
| Full Name | CD83 molecule |
| Calculated molecular weight | 205 aa, 23 kDa |
| GenBank accession number | BC030830 |
| Gene Symbol | CD83 |
| Gene ID (NCBI) | 9308 |
| ENSEMBL Gene ID | ENSG00000112149 |
| RRID | AB_2920522 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
| Form | |
| Form | Liquid |
| Purification Method | The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| UNIPROT ID | Q01151 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD83 antibody PE-65171 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
