• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HCT 116 cells using CD81 Recombinant monoclonal antibody (84644-1-RR)

    WB analysis of HCT 116 using 84644-1-RR

    WB result of CD81 antibody (84644-1-RR; 1:1000; room temperature for 1.5 hours) with wild-type and CD81 knockout HCT 116 cells.

  • Western Blot (WB) analysis of various lysates using CD81 Recombinant antibody (84644-1-RR)

    WB analysis using 84644-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84644-1-RR (CD81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD81 Recombinant monoclonal antibody (84644-1-RR)

    IF Staining of Jurkat using 84644-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD81 antibody (84644-1-RR, Clone: 241722B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84644-1-RR

    Affinity and Kinetic Characterization of 84644-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84644-1-RR against Human CD81 were performed. The affinity constant is 0.602 nM.

  • Featured Product
  • KD/KO Validated

CD81 Recombinant monoclonal antibody

CD81 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84644-1-RR
Clone No.241722B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

241722B8, 26 kDa cell surface protein TAPA-1, CD81 antigen, CD81 molecule, S5.7

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, HCT 116 cells, Ramos cells, Daudi cells, Jurkat cells, THP-1 cells, K-562 cells
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84644-1-RR targets CD81 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1036

Product name: recombinant human CD81 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 113-201 aa of NM_004356.4

Sequence: FVNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFSGK

相同性解析による交差性が予測される生物種
Full Name CD81 molecule
Calculated molecular weight26 kDa
Observed molecular weight20-25 kDa
GenBank accession numberNM_004356.4
Gene Symbol CD81
Gene ID (NCBI) 975
ENSEMBL Gene IDENSG00000110651
RRIDAB_3672103
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP60033
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Protocols

Product Specific Protocols
IF protocol for CD81 antibody 84644-1-RRDownload protocol
WB protocol for CD81 antibody 84644-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...