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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD79b Recombinant antibody (84523-4-RR)

    WB analysis using 84523-4-RR

    Raji cells were subjected to SDS PAGE followed by western blot with 84523-4-RR (CD79b antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD79b Recombinant antibody (84523-4-RR)

    WB analysis using 84523-4-RR

    Mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84523-4-RR (CD79b antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD79b Recombinant antibody (84523-4-RR)

    WB analysis using 84523-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84523-4-RR (CD79b antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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CD79b Recombinant antibody

CD79b Recombinant Antibody for WB, ELISA
Cat No. 84523-4-RR
Clone No.241850G7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Immunoglobulin-associated B29 protein, Ig-beta, Igb, B-cell-specific glycoprotein B29, B-cell antigen receptor complex-associated protein beta chain

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, mouse spleen tissue, Ramos cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84523-4-RR targets CD79b in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name CD79B antigen
Calculated molecular weight26 kDa
Observed molecular weight36-40 kDa
GenBank accession numberNM_008339.3
Gene Symbol Cd79b
Gene ID (NCBI) 15985
RRIDAB_3672031
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP15530
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD79 molecule encompasses two transmembrane proteins, CD79a and CD79b, which form a disulfide-linked heterodimer and are members of the immunoglobulin (Ig) gene superfamily (PMID: 2033258; 1375264; 2304550). CD79b molecule (also known as B29, IGB, AGM6, and Igbeta) is expressed almost exclusively on B cells and B-cell neoplasms, influencing antigen internalization and increasing antigen presentation efficiency (PMID: 7552998). CD79b mutation could be a key biomarker for DLBCL disease progression (PMID: 36182550).

Protocols

Product Specific Protocols
WB protocol for CD79b antibody 84523-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols