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Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD73 Rabbit Recombinant Antibody (98199-1-RR)

    FC experiment of mouse splenocytes using 98199-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD73 Rabbit Recombinant Antibody (98199-1-RR, Clone: 241541G9) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD73 Rabbit Recombinant Antibody (98199-1-RR)

    FC experiment of mouse splenocytes using 98199-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD73 Rabbit Recombinant Antibody (98199-1-RR, Clone: 241541G9) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98199-1-RR

    Affinity and Kinetic Characterization of 98199-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98199-1-RR against Mouse CD73 were performed. The affinity constant is below 1 pM.

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Anti-Mouse CD73 Rabbit Recombinant Antibody

CD73 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98199-1-RR
Clone No.241541G9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Ecto-5'-nucleotidase, EC:3.1.3.99, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98199-1-RR targets CD73 in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name 5' nucleotidase, ecto
Calculated molecular weight64kDa
GenBank accession numberNM_011851.4
Gene Symbol Nt5e
Gene ID (NCBI) 23959
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ61503
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for CD73 antibody 98199-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols