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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using CD72 Polyclonal antibody (13469-1-AP)

    WB analysis of mouse testis using 13469-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13469-1-AP (CD72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

CD72 Polyclonal antibody

CD72 Polyclonal Antibody for WB, ELISA
Cat No. 13469-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Lyb-2, LYB2, Lyb 2, CD72b, CD72 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13469-1-AP targets CD72 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4262

Product name: Recombinant human CD72 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 116-359 aa of BC030227

Sequence: VRYLQVSQQLQQTNRVLEVTNSSLRQQLRLKITQLGQSAEDLQGSRRELAQSQEALQVEQRAHQAAEGQLQACQADRQKTKETLQSEEQQRRALEQKLSNMENRLKPFFTCGSADTCCPSGWIMHQKSCFYISLTSKNWQESQKQCETLSSKLATFSEIYPQSHSYYFLNSLLPNGGSGNSYWTGLSSNKDWKLTDDTQRTRTYAQSSKCNKVHKTWSWWTLESESCRSSLPYICEMTAFRFPD

相同性解析による交差性が予測される生物種
Full Name CD72 molecule
Calculated molecular weight 359 aa, 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC030227
Gene Symbol CD72
Gene ID (NCBI) 971
RRIDAB_10638306
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP21854
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

Protocols

Product Specific Protocols
WB protocol for CD72 antibody 13469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols