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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD71 Recombinant antibody (84766-4-RR)

    WB analysis using 84766-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84766-4-RR (Transferrin R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD71 Recombinant antibody (84766-4-RR)

    IF Staining of RAW 264.7 using 84766-4-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD71 antibody (84766-4-RR, Clone: 242122B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84766-4-RR

    Affinity and Kinetic Characterization of 84766-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84766-4-RR against Mouse CD71 were performed. The affinity constant is 1.04 nM.

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CD71 Recombinant antibody

CD71 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84766-4-RR
Clone No.242122B2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Tfrc, transferrin receptor, TR, TFR1, TFR

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRAW 264.7 cells, mouse placenta tissue
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84766-4-RR targets CD71 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name transferrin receptor
Calculated molecular weight86kDa
Observed molecular weight85-100 kDa
GenBank accession numberNM_011638.4
Gene Symbol CD71
Gene ID (NCBI) 22042
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ62351
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
WB protocol for CD71 antibody 84766-4-RRDownload protocol
IF protocol for CD71 antibody 84766-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols