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Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse bone marrow cells using Anti-Mouse CD71 (RI7217) Rat IgG2a Recombinant Ant (65654-1-TR)

    FC experiment of mouse bone marrow cells using 65654-1-TR (same clone as 65654-1-PBS)

    1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 (RI7217) Rat IgG2a RecAb (65654-1-TR, Clone: RI7217) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse TER-119. Cells were not fixed. This data was developed using the same antibody clone with 65654-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of mouse bone marrow cells using Anti-Mouse CD71 (RI7217) Rat IgG2a Recombinant Ant (65654-1-TR)

    FC experiment of mouse bone marrow cells using 65654-1-TR (same clone as 65654-1-PBS)

    1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD71 (RI7217) Rat IgG2a RecAb (65654-1-TR, Clone: RI7217) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed. This data was developed using the same antibody clone with 65654-1-PBS in a different storage buffer formulation.

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Anti-Mouse CD71 (RI7217) Rat IgG2a Recombinant Antibody, PBS Only

CD71 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65654-1-PBS
Clone No.RI7217

Host / Isotype

Rat / IgG2a

Reactivity

human

Applications

FC

Synonyms

Tfrc, TFR, TFR1, TR, transferrin receptor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65654-1-PBS targets CD71 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rat / IgG2a
Class Recombinant
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name transferrin receptor
GenBank accession numberBC054522
Gene Symbol CD71
Gene ID (NCBI) 22042
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule, creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.