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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD71 Polyclonal antibody (32333-1-AP)

    WB analysis using 32333-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32333-1-AP (CD71 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using CD71 Polyclonal antibody (32333-1-AP)

    IHC staining of mouse placenta using 32333-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 32333-1-AP (CD71 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using CD71 Polyclonal antibody (32333-1-AP)

    IHC staining of mouse placenta using 32333-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 32333-1-AP (CD71 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD71 Polyclonal antibody (32333-1-AP)

    IF Staining of RAW 264.7 using 32333-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD71 antibody (32333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD71 Polyclonal antibody

CD71 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 32333-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Tfrc, TFR, TFR1, TR, transferrin receptor

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRAW 264.7 cells, mouse brain tissue, mouse spleen tissue, human placenta tissue
Positive IHC detected inmouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32333-1-AP targets CD71 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Eg1064

Product name: Recombinant Mouse Transferrin R protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 89-763 aa of NM_011638.4

Sequence: CKRVEQKEECVKLAETEETDKSETMETEDVPTSSRLYWADLKTLLSEKLNSIEFADTIKQLSQNTYTPREAGSQKDESLAYYIENQFHEFKFSKVWRDEHYVKIQVKSSIGQNMVTIVQSNGNLDPVESPEGYVAFSKPTEVSGKLVHANFGTKKDFEELSYSVNGSLVIVRAGEITFAEKVANAQSFNAIGVLIYMDKNKFPVVEADLALFGHAHLGTGDPYTPGFPSFNHTQFPPSQSSGLPNIPVQTISRAAAEKLFGKMEGSCPARWNIDSSCKLELSQNQNVKLIVKNVLKERRILNIFGVIKGYEEPDRYVVVGAQRDALGAGVAAKSSVGTGLLLKLAQVFSDMISKDGFRPSRSIIFASWTAGDFGAVGATEWLEGYLSSLHLKAFTYINLDKVVLGTSNFKVSASPLLYTLMGKIMQDVKHPVDGKSLYRDSNWISKVEKLSFDNAAYPFLAYSGIPAVSFCFCEDADYPYLGTRLDTYEALTQKVPQLNQMVRTAAEVAGQLIIKLTHDVELNLDYEMYNSKLLSFMKDLNQFKTDIRDMGLSLQWLYSARGDYFRATSRLTTDFHNAEKTNRFVMREINDRIMKVEYHFLSPYVSPRESPFRHIFWGSGSHTLSALVENLKLRQKNITAFNETLFRNQLALATWTIQGVANALSGDIWNIDNEF

相同性解析による交差性が予測される生物種
Full Name transferrin receptor
Calculated molecular weight86kDa
Observed molecular weight90-100 kDa
GenBank accession numberNM_011638.4
Gene Symbol CD71
Gene ID (NCBI) 22042
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ62351
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
IF protocol for CD71 antibody 32333-1-APDownload protocol
IHC protocol for CD71 antibody 32333-1-APDownload protocol
WB protocol for CD71 antibody 32333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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