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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD7 (4H9)

CD7 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

4H9

Cat no : 65203-1-Ig

Synonyms

CD7, CD7 molecule, GP40, LEU 9, T cell antigen CD7, T cell leukemia antigen, T cell surface antigen Leu 9, Tp40, TP41

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using 65203-1-Ig

    FC experiment of human PBMCs using 65203-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD7 (65203-1-Ig, Clone:4H9) or Mouse IgG2a Isotype Control, and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:500, then CL405 Anti-Human CD4. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65203-1-Ig

    FC experiment of human PBMCs using 65203-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD7 (65203-1-Ig, Clone:4H9) or Mouse IgG2a Isotype Control, and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD7 Ready-To-Use IHC Kit. CD7 primary antibody included.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65203-1-Ig targets CD7 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen T-acute lymphoblastic leukemia cells 相同性解析による交差性が予測される生物種
Full Name CD7 molecule
Calculated molecular weight 240 aa, 25 kDa
GenBank accession numberBC009293
Gene symbol CD7
Gene ID (NCBI) 924
RRIDAB_2881485
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for CD7 antibody 65203-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols