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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD7 Polyclonal antibody

CD7 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11320-1-AP

Synonyms

CD7, CD7 molecule, GP40, LEU 9, T cell antigen CD7, T cell leukemia antigen, T cell surface antigen Leu 9, Tp40, TP41

Validation Data Gallery

Applications:
  • IHC staining of human tonsillitis using 11320-1-AP

    IHC staining of human tonsillitis using 11320-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11320-1-AP (CD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included
  • IHC staining of human tonsillitis using 11320-1-AP

    IHC staining of human tonsillitis using 11320-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11320-1-AP (CD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included
  • IHC staining of human lung cancer using 11320-1-AP

    IHC staining of human lung cancer using 11320-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11320-1-AP (CD7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included
  • IHC staining of human lung cancer using 11320-1-AP

    IHC staining of human lung cancer using 11320-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11320-1-AP (CD7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included
  • IF Staining of human tonsillitis using 11320-1-AP

    IF Staining of human tonsillitis using 11320-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD7 antibody (11320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human tonsillitis using 11320-1-AP

    IF Staining of human tonsillitis using 11320-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD7 antibody (11320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD7 Ready-To-Use IHC Kit. CD7 primary antibody included.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11320-1-AP targets CD7 in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD7 fusion protein Ag0486 相同性解析による交差性が予測される生物種
Full Name CD7 molecule
Calculated molecular weight 240 aa, 25 kDa
GenBank accession numberBC009293
Gene symbol CD7
Gene ID (NCBI) 924
RRIDAB_2877758
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD7 is a 40 kD type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.

Protocols

Product Specific Protocols
IHC protocol for CD7 antibody 11320-1-APDownload protocol
IF protocol for CD7 antibody 11320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols