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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD69 Polyclonal antibody (10803-1-AP)

    WB analysis using 10803-1-AP (same clone as 10803-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 10803-1-AP (CD69 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD69 Polyclonal antibody (10803-1-AP)

    IHC staining of human tonsillitis using 10803-1-AP (same clone as 10803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD69 Polyclonal antibody (10803-1-AP)

    IHC staining of human tonsillitis using 10803-1-AP (same clone as 10803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD69 Polyclonal antibody (10803-1-AP)

    IHC staining of human tonsillitis using 10803-1-AP (same clone as 10803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD69 Polyclonal antibody (10803-1-AP)

    IHC staining of human tonsillitis using 10803-1-AP (same clone as 10803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD69 Polyclonal antibody, PBS Only (10803-1-PBS)

    IF Staining of human tonsillitis using 10803-1-PBS

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD69 antibody (10803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD69 Polyclonal antibody, PBS Only (10803-1-PBS)

    IF Staining of human tonsillitis using 10803-1-PBS

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD69 antibody (10803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD69 Polyclonal antibody (10803-1-AP)

    IF Staining of human tonsillitis using 10803-1-AP (same clone as 10803-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD69 antibody (10803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD69 Polyclonal antibody (10803-1-AP)

    IF Staining of human tonsillitis using 10803-1-AP (same clone as 10803-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD69 antibody (10803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10803-1-PBS in a different storage buffer formulation.

CD69 Polyclonal antibody, PBS Only

CD69 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 10803-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

Activation inducer molecule, AIM, BL AC/P26, BL-AC/P26, CD69 molecule

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10803-1-PBS targets CD69 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1094

Product name: Recombinant human CD69 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-199 aa of BC007037

Sequence: MSSENCFVAENSSLHPESGQENDATSPHFSTRHEGSFQVPVLCAVMNVVFITILIIALIALSVGQYNCPGQYTFSMPSDSHVSSCSEDWVGYQRKCYFISTVKRSWTSAQNACSEHGATLAVIDSEKDMNFLKRYAGREEHWVGLKKEPGHPWKWSNGKEFNNWFNVTGSDKCVFLKNTEVSSMECEKNLYWICNKPYK

相同性解析による交差性が予測される生物種
Full Name CD69 molecule
Calculated molecular weight 23 kDa
Observed molecular weight 28 kDa, 32 kDa
GenBank accession numberBC007037
Gene Symbol CD69
Gene ID (NCBI) 969
RRIDAB_2074965
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07108
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD69, also known as activation inducer molecule (AIM) or early activation antigen (EA1), is a cell surface glycoprotein expressed shortly after activation on T lymphocytes and other cells of haematopoietic origin, including natural killer cells. It is a disulfide-linked homodimer composed of two chains belonging to the type II integral protein with a C-type lectin domain. CD69 is involved in lymphocyte proliferation and functions as a signal-transmitting receptor in lymphocytes, natural killer cells, and platelets.

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