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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD64 Polyclonal antibody (32060-1-AP)

    WB analysis using 32060-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32060-1-AP (CD64 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD64 Polyclonal antibody (32060-1-AP)

    IHC staining of mouse spleen using 32060-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32060-1-AP (CD64 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD64 Polyclonal antibody (32060-1-AP)

    IHC staining of mouse spleen using 32060-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32060-1-AP (CD64 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CD64 Polyclonal antibody

CD64 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32060-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

Fcg1, Fc-gamma RI, Fcgr1, FcRI, High affinity immunoglobulin gamma Fc receptor I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJ774A.1 cells, RAW 264.7 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32060-1-AP targets CD64 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD64 fusion protein Eg2107 相同性解析による交差性が予測される生物種
Full Name Fc receptor, IgG, high affinity I
Calculated molecular weight45 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_010186.5
Gene Symbol Fcgr1
Gene ID (NCBI) 14129
RRIDAB_3670181
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP26151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.

Protocols

Product Specific Protocols
WB protocol for CD64 antibody 32060-1-APDownload protocol
IHC protocol for CD64 antibody 32060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols