Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-65123 targets CD62L (L-Selectin) in applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | C3H/eb mouse B lymphoma 38C-13 相同性解析による交差性が予測される生物種 |
| Full Name | selectin, lymphocyte |
| GenBank accession number | BC052681 |
| Gene Symbol | CD62L |
| Gene ID (NCBI) | 20343 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 488 nm / 515 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P18337 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
L-selectin, also known as SELL or CD62L, is a member of the selectin family of adhesion molecules that includes E-selectin and P-selectin (PMID: 2663882, 2473156, 1382078). L-selectin is a protein with the calculated molecular mass of 42 kDa but actually 95-105 kDa on neutrophils and 74 kDa on lymphocytes because of the highly glycosylation (PMID: 1694883, 1695155). L-selectin is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). L-selectin mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and are also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).