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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Anti-Human CD61 (VIPL2)

CD61 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

VIPL2

Cat no : FITC-65185

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3

Validation Data Gallery

Applications:
  • FC experiment of human peripheral blood platelets using FITC-65185

    FC experiment of human peripheral blood platelets using FITC-65185

    1X10^6 human peripheral blood platelets were surface stained with 4.00 ul/test FITC Anti-Human CD61 (FITC-65185, Clone:VIPL2) (red) or isotype control antibody (blue). Cells were not fixed.

Tested Applications

Positive FC detected inhuman peripheral blood platelets
Planning an IHC experiment? We recommend our IHCeasy ITGB3/CD61 Ready-To-Use IHC Kit. ITGB3/CD61 primary antibody included.

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 4 µl per 10^6 cells in a 100 µl suspension or 4 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

FITC-65185 targets CD61 in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Reactivity Human, Non-human primates
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Calculated molecular weight 788 aa, 87 kDa
GenBank accession numberBC127666
Gene symbol ITGB3
Gene ID (NCBI) 3690
RRIDAB_2883819
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm / 520 nm
Form Liquid
Purification Method The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD61 is a 110-kDa membrane glycoprotein that is also termed integrin β3 chain or GPIIIa. CD61 can associate with integrin αV chain (CD51) or integrin αIIb chain (CD41, GPIIb) to form heterodimeric receptors for cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, plasminogen, prothrombin, thrombospondin, vitronectin and von Willebrand factor (PMID: 7694604; 9251239; 2018971; 20628538). Integrin αVβ3 (CD51-CD61) is present on endothelium, smooth muscle, osteoclasts, platelets, some fibroblasts, some activated leukocytes and tumor cells (PMID: 7694604). GPllb-llla (CD41-CD61) is restricted in distribution to platelets and cells of megakaryoblastic potential and is required for platelet aggregation (PMID: 2018971; 16322781).

Protocols

Product Specific Protocols
FC protocol for FITC CD61 antibody FITC-65185Download protocol
Standard Protocols
Click here to view our Standard Protocols