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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD6 Recombinant monoclonal antibody (84508-5-RR)

    WB analysis of Jurkat using 84508-5-RR (same clone as 84508-5-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 84508-5-RR (CD6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84508-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD6 Recombinant monoclonal antibody (84508-5-RR)

    IHC staining of human tonsillitis using 84508-5-RR (same clone as 84508-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84508-5-RR (CD6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84508-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD6 Recombinant monoclonal antibody (84508-5-RR)

    IHC staining of human tonsillitis using 84508-5-RR (same clone as 84508-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84508-5-RR (CD6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84508-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD6 Recombinant monoclonal antibody (84508-5-RR)

    IF Staining of Jurkat using 84508-5-RR (same clone as 84508-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD6 antibody (84508-5-RR, Clone: 241965D11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84508-5-PBS in a different storage buffer formulation.

CD6 Recombinant monoclonal antibody, PBS Only

CD6 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84508-5-PBS
Clone No.241965D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

241965D11, CD6 molecule, Soluble CD6, T12, T-cell differentiation antigen CD6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84508-5-PBS targets CD6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2079

Product name: Recombinant Human CD6 protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 18-398 aa of BC033755

Sequence: HPSPAPPDQLNTSSAESELWEPGERLPVRLTNGSSSCSGTVEVRLEASWEPACGALWDSRAAEAVCRALGCGGAEAASQLAPPTPELPPPPAAGNTSVAANATLAGAPALLCSGAEWRLCEVVEHACRSDGRRARVTCAENRALRLVDGGGACAGRVEMLEHGEWGSVCDDTWDLEDAHVVCRQLGCGWAVQALPGLHFTPGRGPIHRDQVNCSGAEAYLWDCPGLPGQHYCGHKEDAGAVCSEHQSWRLTGGADRCEGQVEVHFRGVWNTVCDSEWYPSEAKVLCQSLGCGTAVERPKGLPHSLSGRMYYSCNGEELTLSNCSWRFNNSNLCSQSLAARVLCSASRSLHNLSTPEVPASVQTVTIESSVTVKIENKESRE

相同性解析による交差性が予測される生物種
Full Name CD6 molecule
Observed molecular weight140 kDa
GenBank accession numberBC033755
Gene Symbol CD6
Gene ID (NCBI) 923
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP30203
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD6 is a type I transmembrane glycoprotein that belongs to the scavenger receptor cysteine-rich superfamily (PMID: 21880988). It is composed of an extracellular region, which consists of three tandem SRCR domains, a transmembrane region, and a cytoplasmic tail devoid of intrinsic catalytic activity but harboring several phosphorylatable residues suitable for intracellular signal transduction (PMID: 38139340; 23711376). CD6 is expressed by T cells, medullary thymocytes, a subset of B cells and NK cells, and in some cells of the brain (PMID: 1919444; 21178331). CD6 plays a role in lymphocyte activation, proliferation, and survival processes via interaction with its endogenous ligands.

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