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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD6 Polyclonal antibody (33214-1-AP)

    WB analysis using 33214-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33214-1-AP (CD6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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CD6 Polyclonal antibody

CD6 Polyclonal Antibody for WB, ELISA
Cat No. 33214-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD6 molecule, Soluble CD6, T12, T-cell differentiation antigen CD6, TP120

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHuT 78 cells, human PBMCs

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

33214-1-AP targets CD6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD6 fusion protein Ag40035 相同性解析による交差性が予測される生物種
Full Name CD6 molecule
Calculated molecular weight 668 aa, 72 kDa
Observed molecular weight120-130 kDa
GenBank accession numberBC078669
Gene Symbol CD6
Gene ID (NCBI) 923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP30203
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD6 is a type I transmembrane glycoprotein that belongs to the scavenger receptor cysteine-rich superfamily (PMID: 21880988). It is composed of an extracellular region, which consists of three tandem SRCR domains, a transmembrane region, and a cytoplasmic tail devoid of intrinsic catalytic activity but harboring several phosphorylatable residues suitable for intracellular signal transduction (PMID: 38139340; 23711376). CD6 is expressed by T cells, medullary thymocytes, a subset of B cells and NK cells, and in some cells of the brain (PMID: 1919444; 21178331). CD6 plays a role in lymphocyte activation, proliferation, and survival processes via interaction with its endogenous ligands.

Protocols

Product Specific Protocols
WB protocol for CD6 antibody 33214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols