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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD58 (TS2/9) Mouse IgG1 Recombinant Antibody

CD58 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS2/9

Cat no : 65517-1-MR

Synonyms

Ag3, CD58, CD58 molecule, FLJ23181, FLJ43722, LFA 3, LFA3, Surface glycoprotein LFA 3

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using 65517-1-MR

    FC experiment of human PBMCs using 65517-1-MR

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD58 Mouse Recombinant Antibody (65517-1-MR, Clone:TS2/9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

  • FC experiment of human PBMCs using 65517-1-MR

    FC experiment of human PBMCs using 65517-1-MR

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD58 Mouse Recombinant Antibody (65517-1-MR, Clone:TS2/9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

  • FC experiment of human PBMCs using 65517-1-MR

    FC experiment of human PBMCs using 65517-1-MR

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD58 Mouse Recombinant Antibody (65517-1-MR, Clone:TS2/9) (red), or 0.1 ug Anti-Human CD58 Mouse Monoclonal Antibody (CloneTS2/9) (65193-1-Ig in green or from another vendor in pink), or Mouse IgG1 Isotype Control (blue), and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65517-1-MR targets CD58 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Immunogen Human cytolytic T cells 相同性解析による交差性が予測される生物種
Full Name CD58 molecule
Calculated molecular weight 28 kDa
GenBank accession numberBC005930
Gene symbol CD58
Gene ID (NCBI) 965
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.

Protocols

Product Specific Protocols
FC protocol for CD58 antibody 65517-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols