Validation Data Gallery
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Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65193-1-Ig targets CD58 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Human cytolytic T cells 相同性解析による交差性が予測される生物種 |
| Full Name | CD58 molecule |
| Calculated molecular weight | 28 kDa |
| GenBank accession number | BC005930 |
| Gene Symbol | CD58 |
| Gene ID (NCBI) | 965 |
| RRID | AB_2918463 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19256 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD58 antibody 65193-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
