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Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse bone marrow cells using Anti-Mouse NCAM1/CD56 Rabbit Recombinant Antibody (98118-1-RR)

    FC experiment of mouse bone marrow cells using 98118-1-RR (same clone as 98118-1-PBS)

    1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD56 Rabbit Recombinant Antibody (98118-1-RR, Clone: 240936A4) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98118-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of mouse bone marrow cells using Anti-Mouse NCAM1/CD56 Rabbit Recombinant Antibody (98118-1-RR)

    FC experiment of mouse bone marrow cells using 98118-1-RR (same clone as 98118-1-PBS)

    1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD56 Rabbit Recombinant Antibody (98118-1-RR, Clone:240936A4) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98118-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98118-1-RR

    Affinity and Kinetic Characterization of 98118-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98118-1-RR against Mouse CD56 were performed. The affinity constant is 9.50 nM.

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Anti-Mouse NCAM1/CD56 Rabbit Recombinant Antibody, PBS Only

NCAM1/CD56 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98118-1-PBS
Clone No.240936A4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Ncam1, CD56, 240936A4, NCAM 1, Ncam

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98118-1-PBS targets NCAM1/CD56 in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name neural cell adhesion molecule 1
Calculated molecular weight119 kDa
Gene Symbol Ncam1
Gene ID (NCBI) 17967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13595-1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56, NCAM, and MSK39) is a membrane-bound cell surface glycoprotein and a member of the immunoglobulin supergene family, which induces cell-to-cell interactions during embryonic development, cell migration, and organogenesis (PMID: 8013635; 1855476; 1391354). Mouse natural killer (NK) cells do not express the murine homolog of CD56 (PMID: 17100875). The molecular weight of NCAM1 changes from the 120 kDa isoform in normal tissue to the 140/180 kDa isoforms in tumors (PMID: 10086383).