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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD47 Polyclonal antibody

CD47 Polyclonal Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-29555

Synonyms

CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6

Validation Data Gallery

Applications:
  • FC experiment of HUVEC using CL488-29555

    FC experiment of HUVEC using CL488-29555

    1X10^6 HUVEC cells were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Human CD47 (CL488-29555) (red), or 0.25 ug Control Antibody. Cells were not fixed.

Tested Applications

Positive FC detected inHUVEC cells
For other applications, we recommend the unconjugated version of this antibody, 29555-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-29555 targets CD47 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name CD47 molecule
Calculated molecular weight 323 aa, 35 kDa
GenBank accession numberBC010016
Gene symbol CD47
Gene ID (NCBI) 961
RRIDAB_2919215
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD47 antibody CL488-29555Download protocol
Standard Protocols
Click here to view our Standard Protocols