Validation Data Gallery
Tested Applications
| Positive FC detected in | human peripheral blood leukocyte, transfected CHO cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98531-5-RR targets CD45RB/RAB in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| Calculated molecular weight | 147 kDa |
| GenBank accession number | NM_002838.4 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08575-3 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
CD45RB and CD45RAB are specific isoforms of CD45, a receptor-type protein tyrosine phosphatase (PTP) found on the surface of all nucleated hematopoietic cells, except mature erythrocytes and platelets. They serve as essential phenotypic markers to distinguish naïve T lymphocytes from antigen-experienced memory/effector T cells. CD45RB and CD45RAB designations refer to specific isoforms of CD45 generated through alternative splicing of three variable exons (4, 5, and 6) in the PTPRC gene. These exons encode extracellular domains A, B, and C, respectively, which contain O-linked glycosylation sites that affect the molecule's size, charge, and ligand-binding properties. This antibody can detect CD45RB (contains exons B only) and CD45RAB (contains both exons A and B), but not CD45RABC (contain all three exons) or CD45RBC (contain exons B and C).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD45RB/RAB antibody 98531-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |