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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD45 (2D1)

CD45 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

2D1

Cat no : 65082-1-Ig

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY 5, LY5, PTPRC, RPTPC, T200

Validation Data Gallery

Applications:
  • FC experiment of human peripheral blood lymphocytes using 65082-1-Ig

    FC experiment of human peripheral blood lymphocytes using 65082-1-Ig

    1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD45 (65082-1-Ig, Clone: 2D1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65082-1-Ig targets CD45 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human PBMC 相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
RRIDAB_2918385
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Protocols

Product Specific Protocols
FC protocol for CD45 antibody 65082-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols