• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of rat spleen tissue using CD44 Recombinant antibody (84369-5-RR)

    WB analysis of rat spleen using 84369-5-RR (same clone as 84369-5-PBS)

    rat spleen tissue was subjected to SDS PAGE followed by western blot with 84369-5-RR (CD44 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84369-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD44 Recombinant antibody (84369-5-RR)

    IHC staining of rat spleen using 84369-5-RR (same clone as 84369-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84369-5-RR (CD44 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84369-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD44 Recombinant antibody (84369-5-RR)

    IHC staining of rat spleen using 84369-5-RR (same clone as 84369-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84369-5-RR (CD44 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84369-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NR8383 cells using CD44 Recombinant antibody (84369-5-RR)

    IF Staining of NR8383 using 84369-5-RR (same clone as 84369-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NR8383 cells using CD44 antibody (84369-5-RR, Clone: 241648D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84369-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84369-5-RR

    Affinity and Kinetic Characterization of 84369-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84369-5-RR against Rat CD44 were performed. The affinity constant is 0.332 nM.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Rat CD44 Recombinant antibody, PBS Only

CD44 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84369-5-PBS
Clone No.241648D1

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Hermes antigen, GP90 lymphocyte homing/adhesion receptor, Extracellular matrix receptor III, ECMR-III, CD44 antigen

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84369-5-PBS targets CD44 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Cd44 molecule
Calculated molecular weight40 kDa
Observed molecular weight80 kDa
GenBank accession numberXM_006234631.3
Gene Symbol Cd44
Gene ID (NCBI) 25406
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO70509
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.