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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 555 Anti-Human CD42b (AK2)

CD42b Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

AK2

Cat no : CL555-65163

Synonyms

BSS, CD42B, CD42b alpha, GP1B, GP1BA

Validation Data Gallery

Applications:
  • FC experiment of human peripheral blood platelets using CL555-65163

    FC experiment of human peripheral blood platelets using CL555-65163

    1X10^6 human peripheral blood platelets were surface co-stained with Anti-Human CD41a labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) and 5 ul CoraLite® Plus 555 Anti-Human CD42b (CL555-65163, Clone:AK2) or Mouse IgG1 Isotype Control. Cells were not fixed.

Tested Applications

Positive FC detected inhuman peripheral blood platelets
For other applications, we recommend the unconjugated version of this antibody, 65163-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL555-65163 targets CD42b in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Human platelets 相同性解析による交差性が予測される生物種
Full Name glycoprotein Ib (platelet), alpha polypeptide
Calculated molecular weight 626 aa, 69 kDa
GenBank accession numberBC027955
Gene symbol GP1BA
Gene ID (NCBI) 2811
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification Method Purified by protein-A affinity chromatography
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 CD42b antibody CL555-65163Download protocol
Standard Protocols
Click here to view our Standard Protocols