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Tested Applications
| Positive FC detected in | mosue splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CPY5-65104 targets CD4 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Mouse CTL clone V4 相同性解析による交差性が予測される生物種 |
| Full Name | CD4 antigen |
| GenBank accession number | BC039137 |
| Gene Symbol | Cd4 |
| Gene ID (NCBI) | 12504 |
| RRID | AB_3673818 |
| Conjugate | PerCP-Cyanine5.5 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 482 nm / 690 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P06332 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PerCP-Cyanine5.5 CD4 antibody CPY5-65104 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |