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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD4 Recombinant antibody (83513-7-RR)

    WB analysis using 83513-7-RR (same clone as 83513-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83513-7-RR (CD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83513-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CD4 Recombinant antibody (83513-7-RR)

    IF Staining of THP-1 using 83513-7-RR (same clone as 83513-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD4 antibody (83513-7-RR, Clone: 240427E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83513-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83513-7-RR

    Affinity and Kinetic Characterization of 83513-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83513-7-RR against Human CD4 were performed. The affinity constant is 0.426 nM.

CD4 Recombinant monoclonal antibody, PBS Only

CD4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83513-7-PBS
Clone No.240427E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

240427E12, CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83513-7-PBS targets CD4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0333

Product name: Recombinant Human CD4 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 26-396 aa of BC025782

Sequence: KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQP

相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP01730
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.